MINIMALLY INVASIVE ULTRASOUND-ASSISTED APPLICATION OF AG/CU NANOCOMPOSITES IN THE TREATMENT OF EXPERIMENTAL GENERALIZED PURULENT PERITONITIS CAUSED BY MULITDRUG-RESISTANT MIXED INFECTION petrmyronov@gmail.com

 

MINIMALLY INVASIVE ULTRASOUND-ASSISTED APPLICATION OF AG/CU NANOCOMPOSITES IN THE TREATMENT OF EXPERIMENTAL GENERALIZED PURULENT PERITONITIS CAUSED BY MULITDRUG-RESISTANT MIXED INFECTION

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Ukraine

Oral or Poster

Generalized peritonitis is a severe complication that often results in death, especially in the presence of multidrug-resistant bacteria. The enhanced antibacterial properties of silver and copper as part of Ag/Cu nanocomposites (Ag/Cu NCs), combined with the physicochemical effects of low-frequency ultrasound (LFUS), can be an excellent pathogenetic basis for treating peritonitis. Aim: to investigate the effectiveness of using minimally invasive LFUS with Ag/Cu NCs in treating experimental generalized purulent peritonitis.

The experimental generalized purulent peritonitis was simulated on white rats using a mixture of multidrug-resistant Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa. A 0.005% solution of Ag/Cu NCs with minimally invasive LFUS was used in the first group, and for control, a 0.05% Chlorhexidine solution was used in the second group. The effectiveness of treatment was assessed by morpho-histological and microbiological parameters, the presence of complications, mortality, and recovery times.

The combined application of minimally invasive LFUS with Ag/Cu NCs contributed to a more rapid elimination of purulent-inflammatory processes in the abdominal cavity (fig.1), reduction of bacterial contamination (fig.2), a decrease in the number of complications (15% in the first group; 60% in the second group), mortality (30% and 60% respectively), a reduction in the number of necessary planned sanitation (2.95±0.25 in the first group; 6.62±1.41 in the second group, p<0.05), and the timing of laparostomy closure (5.17±1.33 and 11.5±1.76 days respectively, p<0.05).

The application of minimally invasive LFUS with Ag/Cu NCs significantly reduces the healing time of experimental generalized purulent peritonitis, the number of complications, and the mortality rate.