TRANSCRIPTOMIC ANALYSIS OF MAGNETIC COMPRESSION ANASTOMOSIS ON IMPROVING GASTRIC-JEJUNAL ANASTOMOTIC HEALING IN RATS

Slot ID

Abstract Title

Author Details

No. of Authors

8

Including the presenting author

Author 1

Yong Zhang zhangyongyang05@163.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 2

Aoran Liu wiaff70qgq42@163.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China *

Author 3

Tianren Wang 847774694@qq.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 4

Chenao Yu 798714115@qq.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 5

Haozhen Xu 2456046928@qq.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 6

Runjia Liang littledoubleplus@163.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 7

Changwei Li 501781503@qq.com The First Affiliated Hospital of Xi’an Jiaotong University Department of Thoracic Surgery Xi’an China

Author 8

Zhenzhen Weng wenGzhEnz18@stu.xjtu.edu.cn Xi’an Jiaotong University Medical Science Center Xi’an China

Author 9

Author 10

Author 11

Author 12

Presenting Author Name

Aoran Liu

Presenting Author Email

wiaff70qgq42@163.com

Presenting Author Country

China

Abstract

Abstract type

Oral or Poster

Introduction *

Suture anastomosis carries high risks of leakage and stenosis. Magnetic compression anastomosis (MCA), utilizing magnetic attraction, significantly reduces complications and improves healing. However, the signaling pathways involved remain unclear. This study explored the molecular pathways underlying improved healing at different MCA stages using a rat gastric-jejunal model.

Material & Method *

Twelve adult male SD rats underwent either MCA (n=6) or suture anastomosis (n=6). Anastomotic tissues were harvested on postoperative day 2 (POD2) and 5 (POD5) for RNA-seq. Differential gene analysis, Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Set Enrichment Analysis (GSEA), and Protein-Protein Interaction (PPI) network analysis identified key pathways and hub genes regulating healing.

Results *

Compared to sutures, MCA showed 133 (65 up, 68 down) and 108 (27 up, 81 down) significantly differentially expressed genes (DEGs) at POD2 and POD5. GO/KEGG indicated POD2 DEGs enriched in arachidonic acid metabolism, glutathione, and NOD-like receptor pathways. POD5 DEGs were enriched in ECM-receptor interaction, focal adhesion, and PI3K-Akt pathways. GSEA further validated MCA at POD2 positively regulating NOD-like receptor signaling but negatively regulating arachidonic acid metabolism and gastric acid secretion. At POD5, MCA positively regulated oxidative phosphorylation but negatively regulated ECM-receptor interaction and focal adhesion. PPI analysis identified Pla2g2a, Pla2g1b, Defa24, Plb1 (POD2) and Cd44, Itgb2, Serpine1, Plaur (POD5) as hub genes.

Conclusion *

MCA improves anastomotic healing by stage-specifically modulating pathways involved in inflammation resolution and mechano-sensing, creating a pro-healing microenvironment. Transcriptomics revealed key hub genes (Pla2g2a, Cd44, etc.) and elucidated the potential molecular mechanisms of enhanced healing with MCA, providing crucial insights.

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International Society of Surgery (ISS)

Société Internationale de Chirurgie (SIC)

TRANSCRIPTOMIC ANALYSIS OF MAGNETIC COMPRESSION ANASTOMOSIS ON IMPROVING GASTRIC-JEJUNAL ANASTOMOTIC HEALING IN RATS wiaff70qgq42@163.com